Genotyping

Genotyping can identify differences in the DNA sequence (i.e genotype) of a sample and which enables researchers to explore genetic variants such as single nucleotide polymorphisms (SNPs) and large structural changes in DNA.

BBS is offering different number of approaches to genotyping, including analysis of single nucleotide polymorphisms (SNPs), variation of long or short tandem repeats and other DNA fingerprinting methods.

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Fragment Analysis

Fragment analysis comprises a series of techniques in which DNA fragments are fluorescent labeled, separated by capillary electrophoresis (CE), and sized by comparison to an internal standard. While DNA sequencing by CE is used to determine the specific base sequence of a particular fragment or gene segment, fragment analysis can provide sizing, relative quantitation, and genotyping information for fluorescent labeled DNA fragments produced by PCR using primers designed for a specific DNA target.

BBS is providing genotyping services using microsatellite markers such as SSR, STR, VNTR etc.

SNP

Genotyping is a method used to determine the genetic makeup of an organism. This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.

Barcode BioSciences SNP Genotyping service is a PCR and Sanger sequencing-based solution that is used to ramp up SNP screening assays and validate SNPs of interest with speed and accuracy.

MLST- Multi Locus Sequence Typing

Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer. For each house-keeping gene, the different sequences present within a bacterial species are assigned as distinct alleles and, for each isolate, the alleles at each of the seven loci define the allelic profile or sequence type (ST).

Each isolate of a species is therefore unambiguously characterised by a series of seven integers which correspond to the alleles at the seven house-keeping loci.

Barcode BioSciences Doctorate qualified scientists are proficient in assigning the allelic profiles.

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